Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96667
Philips GT, et al., Precocious retinal neurons: Pax6 controls timing of differentiation and determination of cell type. Dev Biol. 2005 Mar 15;279(2):308-21
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6343379
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Probe:
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Mash1 probe16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5C:
embryonic day 9.5
(more  )
Specimen
5C:
(close  )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
5D:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Present |
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5D
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Expressing cells were more numerous than in wildtype.
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Specimen
Pax6-/- 20 somites not shown:
embryonic day 9.0; Pax6Sey/ Pax6Sey
(more )
Specimen
Pax6-/- 20 somites not shown:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: optic vesicle |
Present |
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Authors report expression in optic vesicle in mutants as early as at the 20 somite stage.
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Specimen
5G:
embryonic day 10.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Absent |
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5G
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Expression disappeared by E10.5
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Specimen
5H:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
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5H
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Expressing cells were more numerous than in wildtype.
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Specimen
5I:
embryonic day 8.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural plate |
Absent |
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5I
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No expression in anterior neural plate of wild type embryos.
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Specimen
Pax+/- E8.5 not shown:
embryonic day 8.5; Pax6Sey/Pax6 +
(more )
Specimen
Pax+/- E8.5 not shown:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Present |
Regionally restricted |
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Authors report ectopic expression in dorsal forebrain of Pax6 heterogygotes.
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Specimen
5J:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Analysis Tools