Calcatm2.1(cre/EGFP)Rpa
Targeted Allele Detail
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| Symbol: |
Calcatm2.1(cre/EGFP)Rpa |
| Name: |
calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 2.1, Richard D Palmiter |
| MGI ID: |
MGI:6357958 |
| Synonyms: |
CalcafrtCre |
| Gene: |
Calca Location: Chr7:114230713-114235592 bp, - strand Genetic Position: Chr7, 59.99 cM
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| Alliance: |
Calcatm2.1(cre/EGFP)Rpa page
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| Allele Type: |
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Targeted (Conditional ready, Recombinase, Reporter) |
| Mutation: |
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Insertion
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Calcatm2.1(cre/EGFP)Rpa expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting construct was made by inserting a Mt1 gene intron within the open reading frame of mnCre:EGFP, which has a Myc tag (m), followed by a nuclear localization signal (NLS, n), followed by Cre recombinase fused to green fluorescent protein (Cre:GFP), to generate a gene with two exons. Then the second exon was inverted and flanked by a pair of dissimilar frt sites to make a DIO (double-frt inverse open reading frame) construct. Frt-DIO-mnCre:GFP cassette was inserted at the initiation ATG codon in exon 2 of the gene. FLP recombinase inverts the second exon allowing splicing to generate functional Cre recombinase. Frt flanked SV-Neo was removed by crossing with FLPer mice.
(J:269999)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Calca
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Calca Mutation: |
23 strains or lines available
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| Original: |
J:269999 Chen JY, et al., Parabrachial CGRP Neurons Establish and Sustain Aversive Taste Memories. Neuron. 2018 Nov 21;100(4):891-899.e5 |
| All: |
1 reference(s) |
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Analysis Tools
