Gene Expression Data
Assay Details
Assay
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Reference:
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J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358676
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Gene symbol:
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Efna5
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Gene name:
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ephrin A5
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Probe:
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EphrinA5 cDNA4
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5A:
embryonic day 18.5
(more )
Specimen
5A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
5A
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Expression was detected most highly in dorsolateral parts (somatosensory area).
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Specimen
5A':
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
5A'
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The expression domain was shifted and restricted caudally in Emx2 mutant neocortex.
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Specimen
5B:
embryonic day 18.5
(more )
Specimen
5B:
(close )
Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
5B
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Expression was detected most highly in dorsolateral parts (somatosensory area).
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Specimen
5B':
(close )
Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
5B'
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In Pax6 mutants, the expression domain was shifted and restricted rostrally.
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Specimen
7D:
embryonic day 18.5
(more )
Specimen
7D:
(close )
Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
7D
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High expression was centered on the somatosensory area.
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Specimen
7D':
(close )
Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
7D'
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Expression in Emx1 mutants was very similar to wild type.
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Specimen
9C:
embryonic day 18.5; Emx1tm1Jlr/Emx1 +
(more )
Specimen
9C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
9C
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High expression was detected centered on the somatosensory area.
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Specimen
9C':
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
9C'
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Expression was shifted caudally in the Emx double mutants.
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Specimen
9C":
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
9C"
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Expression was shifted caudally in the Emx2 mutants.
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