Tg(CAG-cre)#Ben
Transgene Detail
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| Symbol: |
Tg(CAG-cre)#Ben |
| Name: |
transgene insertion, Rui Benedito |
| MGI ID: |
MGI:6361145 |
| Synonyms: |
Tg(iSuRe-Cre) |
| Transgene: |
Tg(CAG-cre)#Ben Location: unknown
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| Alliance: |
Tg(CAG-cre)#Ben page
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| Transgene Type: |
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Transgenic (Conditional ready, Recombinase, Reporter) |
| Mutation: |
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Insertion
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Tg(CAG-cre)#Ben expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| chicken |
CAG |
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Mutation details: The transgene contains four chicken -globin HS4 insulator sequences (two on each ends) flanking the ubiquitous and strong CAG promoter driving LoxP-flanked (floxed) DNA sequence which has the N-PhiM reporter gene encoding a noncytotoxicand non-fluorescent mutated PhiYFP protein with a nuclear localization signal and a transcription stop signal followed by bright membrane-localized fluorescent reporter (MbTomato) and cre gene separated by 2A cleavage site (T2A; mediates ribosomal skipping), then a WPRE sequence, an SV40 polyA signal. A Frt flanked PGK-neo cassette followed those sequences. After recombination/deletion of the floxed cassette by Cre or CreERT2, MbTomato and Cre protein are constitutively active and permanently expressed. Founder information and line numbers were not available. The pound symbol (#) is used when no line is specified and/or lines are pooled.
(J:278452)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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CAG
(chicken)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:278452 Fernandez-Chacon M, et al., iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications. Nat Commun. 2019 May 22;10(1):2262 |
| All: |
2 reference(s) |
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Analysis Tools
