Celf4em1(icre/ERT2)Zyliu
Endonuclease-mediated Allele Detail
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| Symbol: |
Celf4em1(icre/ERT2)Zyliu |
| Name: |
CUGBP, Elav-like family member 4; endonuclease-mediated mutation 1, Zhiyong Liu |
| MGI ID: |
MGI:6368146 |
| Synonyms: |
Celf4-3xHA-P2A-iCreER-T2A-EGFP |
| Gene: |
Celf4 Location: Chr18:25610689-25887214 bp, - strand Genetic Position: Chr18, 13.92 cM, cytoband B1
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| Alliance: |
Celf4em1(icre/ERT2)Zyliu page
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| Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Celf4em1(icre/ERT2)Zyliu expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: Using CRISPR/Cas9 with single guide RNAs and a targeting vector, the following elements were inserted immediately upstream of the stop codon: a 3x hemagglutinin tag (HA) followed by sequence for self-cleaving oligopeptide P2A fused to a codon improved Cre recombinase and estrogen receptor 1 (human) fusion gene, and sequence for self-cleaving oligopeptide T2A fused to an enhanced green fluorescent protein sequence (EGFP) gene. This allele thus expresses the endogenous peptide, Cre and EGFP under the control of the endogenous promoter.
(J:293995)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Celf4
(mouse)
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| Original: |
J:293995 Li C, et al., Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages. Elife. 2020 Jan 8;9:e50491 |
| All: |
1 reference(s) |
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Analysis Tools
