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Col1a1tm2(tetO-GFP/RNAi:Rpl5)Begg
Targeted Allele Detail
Summary
Symbol: Col1a1tm2(tetO-GFP/RNAi:Rpl5)Begg
Name: collagen, type I, alpha 1; targeted mutation 2, Alan Beggs
MGI ID: MGI:6368192
Synonyms: shRNA Rpl5
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm2(tetO-GFP/RNAi:Rpl5)Begg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:341566
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Knockdown, Reporter)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
  Col1a1tm2(tetO-GFP/RNAi:Rpl5)Begg involves 1 genes/genome features (Rpl5) View all
 
Mutation details: RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Rpl5 into the Col1a1 locus. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a turboGFP sequence (tGFP; a fast-maturating and short-half-life green fluorescent protein variant) and short-hairpin RNA sequence targeting Rpl5 embedded in the MIR30 structure. The vector was then co-electroporated with a FLPe recombinase-expressing vector into the RMCE-ready KH2 ES cells. (J:341566)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  163 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:341566 Kazerounian S, et al., Rpl5-Inducible Mouse Model for Studying Diamond-Blackfan Anemia. Discoveries (Craiova). 2019 Sep 30;7(3):e96
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory