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P2ry12em1(icre/ERT2)Tda
Endonuclease-mediated Allele Detail
Summary
Symbol: P2ry12em1(icre/ERT2)Tda
Name: purinergic receptor P2Y, G-protein coupled 12; endonuclease-mediated mutation 1, Thomas D Arnold
MGI ID: MGI:6379582
Synonyms: P2ry12-CreER, P2Y12-CreER
Gene: P2ry12  Location: Chr3:59123693-59170292 bp, - strand  Genetic Position: Chr3, 29.01 cM
Alliance: P2ry12em1(icre/ERT2)Tda page
Mutation
origin
Strain of Origin:  C57BL/6N
Mutation
description
Allele Type:    Endonuclease-mediated (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
P2ry12em1(icre/ERT2)Tda expression driven by 1 gene
 
Mutation details: The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the P2ry12 locus on chromosome 3. The cas9-RNP complex and a 2A-iCreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the iCreER fusion gene with ~1.5 kbp homology arms) were injected into the pronucleus of C57BL/6N mouse embryos, followed by adoptive embryo transfer. Founder mice and progeny were screened by PCR, and proper genomic integration was verified by Southern blot and genomic sequencing. Founders were bred to C57BL/6J mice for germline transmission. (J:291145)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: P2ry12 (mouse)
Summary of all recombinase alleles driven by P2ry12.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any P2ry12 Mutation:  41 strains or lines available
References
Original:  J:291145 McKinsey GL, et al., A new genetic strategy for targeting microglia in development and disease. Elife. 2020 Jun 23;9:e54590
All:  5 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory