Ripk3 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279204 Ferrando RE, et al., Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J Histochem Cytochem. 2015 Apr;63(4):244-55
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6380490
Gene symbol:	Ripk3
Gene name:	receptor-interacting serine-threonine kinase 3

Reporter:	FLAG
Antibody:	Anti-Flag tag antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 2A: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	Present		2A	Expressed in the cytoplasm.

Specimen 2B: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Present		2B	Expressed in Kupffer cells. Expressed in the cytoplasm.

Specimen 2C: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Present		2C	Expressed in the cytoplasm.

Specimen 2D: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: large intestine crypt of lieberkuhn	Present		2D	Expressed in the cytoplasm.

Specimen 2Q: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	Present		2Q	Expressed in the cytoplasm.

Specimen 2R: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Present		2R	Expressed in Kupffer cells. Expressed in the cytoplasm.

Specimen 2S: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Present		2S	Expressed in the cytoplasm.

Specimen 2T: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: large intestine crypt of lieberkuhn	Present		2T	Expressed in the cytoplasm.

Specimen 3: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Present		3_right	Expressed in Kupffer cells. Expressed in the cytoplasm.

Specimen 9 RIPK3.3xFLAG: postnatal adult; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Image	Note
TS28: spleen	Present		9_right upper right

Specimen not shown: postnatal week 12; Ripk3^tm2.1Geno/Ripk3^tm2.1Geno (more

)

Structure	Level	Pattern	Note
TS28: lung alveolus	Present		Expressed in alveolar macrophages.
TS28: bone marrow	Present	Scattered	Expressed in lymphoid cells.
TS28: spleen	Present	Scattered	Expressed in lymphoid cells.
TS28: pancreatic acinus	Present		Expressed in pancreatic acinar cells.
TS28: lymph node cortex	Present		Expressed in the cortex and para-cortex.
TS28: heart	Present	Regionally restricted	Expressed in the endothelium and intra-vascular leukocytes.
TS28: heart endothelium	Present
TS28: metanephros	Absent
TS28: skin	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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