Reporter:	FLAG
Antibody:	Anti-Flag tag antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: gallbladder epithelium	Present	Widespread	5A

Structure	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	Present		5B	Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Structure	Level	Pattern	Image	Note
TS28: cecum	Present	Regionally restricted	5C	Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5D	Note: non-specific labeling of Leydig cells.

Structure	Level	Pattern	Image	Note
TS28: gallbladder epithelium	Present	Widespread	5Q

Structure	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	Present		5R	Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Structure	Level	Pattern	Image	Note
TS28: cecum	Present	Regionally restricted	5S	Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5T	Note: non-specific labeling of Leydig cells.

Structure	Level	Pattern	Image	Note
TS20: embryo	Strong	Ubiquitous	7A

Structure	Level	Pattern	Image	Note
TS23: embryo	Moderate	Non-Uniform	7B	Moderate to weak expression in most tissues, although there was diminished immunoreactivity in the liver, brain and connective tissue.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present		7C	Expressed in the ganglia, salivary gland, intestine, epidermis and thymus. Also heterogeneous detection in the brain and connective tissues, and moderate to weak expression in the liver and lung.

Structure	Level	Pattern	Image	Note
TS28: spleen	Present		9_right upper left

Structure	Level	Pattern	Image	Note
TS28: large intestine crypt of lieberkuhn	Present			Nuclear expression in the cells of the large intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
TS28: ovary follicle	Present
TS28: lymph node cortex	Present	Regionally restricted		Expressed in the para-cortex of the mesenteric lymph node.

Structure	Level	Pattern	Image	Note
TS26: epidermis	Present
TS26: ganglion	Present
TS26: intestine	Present
TS26: salivary gland	Present
TS26: thymus	Present
TS26: brain	Present			Heterogeneous expression.
TS26: connective tissue	Present			Heterogeneous expression.
TS26: liver	Moderate			Moderate to weak expression.
TS26: lung	Moderate			Moderate to weak expression.