Kif23 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:272586 Suzuki T, et al., Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes. Development. 2019 Feb 15;146(4):dev168146
Assay type:	RT-PCR
MGI Accession ID:	MGI:6383144
Gene symbol:	Kif23
Gene name:	kinesin family member 23

Probe:	Kif23-pA, Kif23-pB
Assay notes:	Quantitative RT-PCR. Gapdh mRNA levels were used for normalization. Expression levels of genes in postnatal week 4 control mice were set to 1.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
1w Control	P w 1	TS28: liver	Present	1 µg; total RNA	involves: 129P2/OlaHsd		Not Specified	The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
1w Cnot3LKO	P w 1	TS28: liver	Present	1 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6 * DBA	Cnot3^tm1.2Tya/Cnot3^tm1.2Tya, Speer6-ps1^{Tg(Alb-cre)21Mgn}/0	Not Specified	Conditional mutant.
4w Control	P w 4	TS28: liver	Present	1 µg; total RNA	involves: 129P2/OlaHsd		Not Specified	The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
4w Cnot3LKO	P w 4	TS28: liver	Present	1 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6 * DBA	Cnot3^tm1.2Tya/Cnot3^tm1.2Tya, Speer6-ps1^{Tg(Alb-cre)21Mgn}/0	Not Specified	Conditional mutant.

Notes:
(a) Transcripts were expressed more highly in the conditional mutant liver compared with control, mainly at 4 weeks of age. The results also showed that transcript level decreased less efficiently from postnatal week 1 to postnatal week 4 in the conditional mutant liver compared to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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