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Tg(Chat-flpo)22Majh
Transgene Detail
Summary
Symbol: Tg(Chat-flpo)22Majh
Name: transgene insertion 22, Marthe Howard
MGI ID: MGI:6387001
Synonyms: ChAT-FlpO
Transgene: Tg(Chat-flpo)22Majh  Location: unknown  
Alliance: Tg(Chat-flpo)22Majh page
Transgene
origin
Strain of Origin:  C57BL/6 x SJL
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Tg(Chat-flpo)22Majh expression driven by 1 gene
 
Mutation details: The 176 kb bacterial artificial chromosome, RP24-256F2, containing the entire Chat and Slc18a3 (VAChT) genes was modified to insert an optimized FLP recombinase (FLPo) followed by a roxP-flanked PGK-prokaryotic gb2 promoter-neoR/kanR cassette under the direction of the Chat promoter. Induction of Dre recombinase removes the kanamycin cassette in recombineering competent bacteria. Next, a 3X Stop is inserted into the Slc18a3 (or VAChT) blocking transcription. (J:101977)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Chat (mouse)
Summary of all recombinase alleles driven by Chat.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory