Antibody:	anti-STk2
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Strong		3A	Expressed in radial glial cells processes and in the nuclei of most precursors.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		3B	Expressed in radial glial cells processes and in some of the neuroblasts.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		3C	Expressed in some of the neuroblasts.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3D

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	3F , 3H	Expressed in the nuclei of the ventricular and subventricular zones.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular layer	Present		3L , 3J	Expressed in both the nuclei of differentiating Tubb3+ neuroblast and most progenitors Tubb3- cells, which is composed of gliogenic and bipotent radial glial cells (75%) and basal progenitors (25%). Expression was absent in the mitotic condensed chromosomes.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	3P , 3N	Broadly expressed in the nuclei of postmitotic neurons (colocalized with Rbfox3) in structures all along the rostro-caudal axis.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Strong		4C , 4A	Coexpressed with Gfap in type B cellular processes (neural stem cells). Also expressed in neurogenic niche cells.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Strong		4E	Coexpressed with Gfap in type B cellular processes (neural stem cells).

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Strong		4F	Coexpressed with Dcx in type A cells (migrating neuroblasts). Expressed in the nuclei, and Dcx in their cell bodies.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Strong		4H , 4J	Coexpressed with Dcx in type A cells (migrating neuroblasts), but not in condensed chromosomes.

Structure	Level	Pattern	Image	Note
TS28: hippocampus layer	Present	Regionally restricted	4M	Coexpressed with Dcx in the subgranular zone.

Structure	Level	Pattern	Image	Note
TS28: hippocampus layer	Present	Regionally restricted	4N	Coexpressed with Ncam1 in the subgranular zone.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		S4B , S4D	Expressed in radial glial cell processes and end-feet at the pial surface. Also expressed in some nuclei of cells distributed throughout the developing neural tube.

Structure	Level	Pattern	Image	Note
TS22: hindbrain lateral wall	Present	Regionally restricted	S4H , S4J , S4L , S4F	Most ventricular zone TUBB3- progenitor cells express ANKK1, whereas the subventricular zone and upper layers present ANKK1+/TUBB3-, ANKK1+/TUBB3+ and some ANKK1-/TUBB3+ cells.
TS22: hindbrain ventricular layer	Present		S4F , S4L , S4H , S4J	Most ventricular zone TUBB3- progenitor cells express ANKK1.

Structure	Level	Pattern	Image	Note
TS25: midbrain	Strong		S4P , S4N	Expressed in both the nuclei of differentiating Tubb3+ neuroblast and most progenitors Tubb3- cells. Intense staining in the basal surface.

Structure	Level	Pattern	Image	Note
TS25: hindbrain lateral wall	Present		S4R , S4T	Expressed in both the nuclei of differentiating Tubb3+ neuroblast and most progenitors Tubb3- cells in the ventricular and subventricular zones.
TS25: hindbrain ventricular layer	Present		S4T , S4R	Expressed in both the nuclei of differentiating Tubb3+ neuroblast and most progenitors Tubb3- cells.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular layer	Present		S5D , S5B	Expressed in progenitors.
TS25: cerebral cortex subventricular zone	Present		S5D , S5B	Expressed in nonepithelial progenitor cells located in a deeper subventricular zone.
TS25: corpus striatum	Present		S5B , S5D	Expressed in the progenitors of the ventricular zone, and also in the secondary proliferative population, which consists of nonepithelial progenitor cells located in a deeper subventricular zone. Expression was absent in the mitotic condensed chromosomes.

Structure	Level	Pattern	Image	Note
TS25: hippocampus ventricular layer	Present		S5H , S5F	Expressed in the nuclei of the progenitor cells and migrating neuroblasts. Expression was absent in the mitotic condensed chromosomes.

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present		S5J , S5L	Expressed mainly in the ventricular zone, although some migrating TUBB3+ cells found throughout the cortical layers were also positive in their nuclei.
TS25: cerebral cortex ventricular layer	Present		S5L , S5J
TS25: cerebral cortex intermediate zone	Present		S5L , S5J	Expressed in tangential migrating interneurons in the lower intermediate zone.