Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32936
Lowe LA, et al., Conserved left-right asymmetry of nodal expression and alterations in murine situs inversus [see comments]. Nature. 1996 May 9;381(6578):158-61
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6392343
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1a:
embryonic day 7.5
(more  )
Note: pre-somite
Specimen
1a:
(close  )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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pre-somite
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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1a
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Specimen
2-3 somite stage not shown:
embryonic day 8.0
(more )
Note: 2-3 somites
Specimen
2-3 somite stage not shown:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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2-3 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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Asymmetric expression seen as early as 2-3 somite stage in small fraction of embryos.
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Specimen
1b:
embryonic day 8.0
(more )
Note: 3-4 somites
Specimen
1b:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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3-4 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
1b
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Authors report progression to asymmetry around the node. Expression becomes stronger on left side of node.
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Specimen
1c:
embryonic day 8.0
(more )
Note: 5-6 somites
Specimen
1c:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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5-6 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
1c
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Authors report progression to asymmetry around the node. Expression becomes stronger on left side of node.
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Specimen
1d:
embryonic day 8.0
(more )
Note: 4-5 somites
Specimen
1d:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Weak |
Regionally restricted |
1d
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Limited expression in left lateral plate mesoderm lateral to the node.
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Specimen
1e:
embryonic day 8.0
(more )
Note: 5-6 somites
Specimen
1e:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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5-6 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Widespread |
1e
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Expression throughout the left lateral plate in most embryos.
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Specimen
1f:
embryonic day 8.5
(more )
Note: 7-8 somites
Specimen
1f:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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7-8 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
1f
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Authors report mostly anterior lateral plate mesoderm expression.
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Specimen
1g:
embryonic day 8.5
(more )
Note: 7-8 somitex
Specimen
1g:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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7-8 somitex
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Specimen
9-10 somite stage not shown:
embryonic day 8.5
(more )
Note: 9-10 somitex
Specimen
9-10 somite stage not shown:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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9-10 somitex
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Specimen
1h:
embryonic day 8.0
(more )
Note: 5-6 somites. Embedded in plastic.
Specimen
1h:
(close )
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Genetic Background:
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involves: BALB/c * Swiss Webster
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Resin/plastic
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Note:
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5-6 somites. Embedded in plastic.
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Specimen
3b (left):
embryonic day 8.0; Dnah11iv/ Dnah11iv
(more )
Note: 6-7 somites
Specimen
3b (left):
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6-7 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3b (left)
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Authors report right lateral plate expression
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Specimen
3b (center-left):
embryonic day 8.0; Dnah11iv/ Dnah11iv
(more )
Note: 6-7 somites
Specimen
3b (center-left):
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6-7 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3b (center-left)
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Authors report right lateral plate expression
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Specimen
3b (center-right):
embryonic day 8.0; Dnah11iv/ Dnah11iv
(more )
Note: 3-4 somites
Specimen
3b (center-right):
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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3-4 somites
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Specimen
3b (right):
embryonic day 8.5; Dnah11iv/ Dnah11iv
(more )
Note: 7-8 somites
Specimen
3b (right):
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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7-8 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3b (right)
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Authors report normal left lateral plate expression.
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Specimen
3c (left):
embryonic day 8.0; Dnah11iv/ Dnah11iv
(more )
Note: 6-7 somites
Specimen
3c (left):
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6-7 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3c (left)
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Varying degrees of expression in both right and left lateral plates
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Specimen
3c (right):
embryonic day 8.0; Dnah11iv/ Dnah11iv
(more )
Note: 6-7 somites
Specimen
3c (right):
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6-7 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3c (right)
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Varying degrees of expression in both right and left lateral plates
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