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Gene Expression Data
Assay Details
Assay
Reference: J:285737 Cui W, et al., MCRS1 is essential for epiblast development during early mouse embryogenesis. Reproduction. 2020 Jan;159(1):1-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6404981
Gene symbol: Sox17
Gene name: SRY (sex determining region Y)-box 17
Antibody: anti-Sox17 (AF1924)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen +/+: embryonic day 3.5 (more )
Note: Triple labeled: green - Sox17; white - Cdx2 (assay MGI:6404960); red - Nanog (assay MGI:6404983). Blastocysts were flushed out at E3.5, then fixed directly for staining.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4_Sox17 Mcrs1+/+ , S4_Merge Mcrs1+/+ Expressed in primitive endoderm.
Specimen +/-: embryonic day 3.5; Mcrs1tm1.1(KOMP)Vlcg/Mcrs1+ (more )
Note: Triple labeled: green - Sox17; white - Cdx2 (assay MGI:6404960); red - Nanog (assay MGI:6404983). Blastocysts were flushed out at E3.5, then fixed directly for staining.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4_Merge Mcrs1+/- , S4_Sox17 Mcrs1+/- Similar as in the wild-type. Expressed in primitive endoderm.
Specimen -/-: embryonic day 3.5; Mcrs1tm1.1(KOMP)Vlcg/Mcrs1tm1.1(KOMP)Vlcg (more )
Note: Triple labeled: green - Sox17; white - Cdx2 (assay MGI:6404960); red - Nanog (assay MGI:6404983). Blastocysts were flushed out at E3.5, then fixed directly for staining.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4_Merge Mcrs1-/- , S4_Sox17 Mcrs1-/- Similar as in the wild-type. Expressed in primitive endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory