Gene Expression Data
Assay Details
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Assay
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Reference:
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J:203477
McDermott JP, et al., Green fluorescence protein driven by the Na,K-ATPase alpha4 isoform promoter is expressed only in male germ cells of mouse testis. J Assist Reprod Genet. 2012 Dec;29(12):1313-25
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6415244
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Gene symbol:
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Atp1a4
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Gene name:
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ATPase, Na+/K+ transporting, alpha 4 polypeptide
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Reporter:
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GFP
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Antibody:
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anti-GFP
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
5D Atp1a4
embryonic day 16.0; Atp1a4tm1Blac/Atp1a4+
(more  )
Specimen
5D Atp1a4
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Atp1a4tm1Blac/Atp1a4+
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: embryo |
Absent |
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5D Atp1a4null(GFP)
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Specimen
7A 7 dpp Apt1a4null(GFP):
postnatal day 7; Atp1a4tm1Blac/Atp1a4 +
(more )
Specimen
7A 7 dpp Apt1a4:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Atp1a4tm1Blac/Atp1a4+
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: testis |
Absent |
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7A Atp1a4null(GFP) 7dpp
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Specimen
7A 18 dpp Apt1a4null(GFP):
postnatal day 18; Atp1a4tm1Blac/Atp1a4 +
(more )
Specimen
7A 18 dpp Apt1a4:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Atp1a4tm1Blac/Atp1a4+
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Age:
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postnatal day 18
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: testis |
Absent |
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7A Atp1a4null(GFP) 18dpp
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Specimen
7A Adult Apt1a4null(GFP):
postnatal adult; Atp1a4tm1Blac/Atp1a4 +
(more )
Specimen
7A Adult Apt1a4:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Atp1a4tm1Blac/Atp1a4+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
Present |
Regionally restricted |
7A Atp1a4null(GFP) Adult
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Expression was localized to the inner region of the seminiferous tubules.
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Specimen
7B Apt1a4null(GFP):
postnatal adult; Atp1a4tm1Blac/Atp1a4 +
(more )
Specimen
7B Apt1a4:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Atp1a4tm1Blac/Atp1a4+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Analysis Tools
