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Uts2bem1(cre)Zak
Endonuclease-mediated Allele Detail
Summary
Symbol: Uts2bem1(cre)Zak
Name: urotensin 2B; endonuclease-mediated mutation 1, Zachary Knight
MGI ID: MGI:6448061
Synonyms: Uts2b2A-Cre, Uts2bcre
Gene: Uts2b  Location: Chr16:27172072-27188989 bp, - strand  Genetic Position: Chr16, 18.93 cM
Alliance: Uts2bem1(cre)Zak page
Mutation
origin
Strain of Origin:  FVB/N
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Uts2bem1(cre)Zak expression driven by 1 gene
 
Mutation details: CRISPR/Cas9 genome editing is used to insert a T2A-Cre cassette immediately upstream of the endogenous stop codon, a 1kb upstream homology arm, and a 2kb downstream homology arm with a TG to GT mutation introduced into the 3' UTR (114-115bp after stop codon) to avoid cleavage by the sgRNA. The T2A oligopeptide mediates ribosomal skipping and is derived from foot-and-mouth disease virus 2A from the insect Thosea asigna virus. T2A permits cre recombinase to be expressed in frame with Uts2b as a separate polypeptide. (J:291579)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Uts2b (mouse)
Summary of all recombinase alleles driven by Uts2b.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Uts2b Mutation:  8 strains or lines available
References
Original:  J:291579 Bai L, et al., Genetic Identification of Vagal Sensory Neurons That Control Feeding. Cell. 2019 Nov 14;179(5):1129-1143.e23
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory