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Gene Expression Data
Assay Details
Assay
Reference: J:278346 Winkler CC, et al., Loss of Shh signaling in the neocortex reveals heterogeneous cell recovery responses from distinct oligodendrocyte populations. Dev Biol. 2019 Aug 1;452(1):55-65
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6448412
Gene symbol: Olig2
Gene name: oligodendrocyte transcription factor 2
Antibody: anti-Olig2
Detection system: Secondary antibody coupled to other - see notes
Assay notes: Visualization was with Opal 650 reagent.
Results
Specimen 2B +/+: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: neocortex region Present Regionally restricted 2B Smo+/+ Expression is in oligodendrocytes in the somatosensory cortex.
Specimen 2B Smo fl/fl: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: neocortex region Present Single cells 2B Smofl/fl The number of positive oligodendrocytes in the somatosensory cortex is significantly reduced and are ventrally-derived in this mutant compared to controls.
Specimen 2D Example 1: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Double labeled: red - Olig2; green Smo (assay MGI:6448415).
Structure Level Pattern Image Note
TS25: neocortex Present Single cells 2D Example 1 Merge , 2D Example 1 Olig2
Specimen 2D Example 2: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Double labeled: red - Olig2; green Smo (assay MGI:6448415).
Structure Level Pattern Image Note
TS25: neocortex Present Single cells 2D Example 2 Olig2 , 2D Example 2 Merge
Specimen 2D Example 3: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Double labeled: red - Olig2; green Smo (assay MGI:6448415).
Structure Level Pattern Image Note
TS25: neocortex Present Single cells 2D Example 3 Olig2 , 2D Example 3 Merge
Specimen 3A Smo+/+: postnatal day 30; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: neocortex Present Single cells 3A Smo+/+
Specimen 3A Smofl/fl: postnatal day 30; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: neocortex Present Single cells 3A Smofl/fl The number of positive oligodendrocytes in the somatosensory cortex is significantly reduced and are ventrally-derived in this mutant compared to controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory