Antibody:	anti-collagen XXVIII (KR43)
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Anti-collagen XXVIII antibody MGI:6452149 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present	Regionally restricted	S1B , S1C	Expression in extracellular matrix of dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present	Regionally restricted	S1E , S1F	Expression in extracellular matrix of dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present	Regionally restricted	S1I , S1H	Expression in extracellular matrix of dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Strong	Regionally restricted	1A	Strong expression in dorsal root ganglion, clearly located outside the neuronal body-satellite glial cell (NB-SGC) unit.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	1B	Expression both around neural cells and in nerve fibers.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	1C	Expression at basal lamina outside neuronal body-satellite glial cell (NB-SGC) unit. Expression around axons emerging from soma of neuron.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	1D	Expression located outside neuronal body-satellite glial cell (NB-SGC) unit. No colocaliztion with Col16a1 expression.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	1F , 1G	Partial colocalization with Lamc1 expression. No expression in connective tissue capsule of dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	1H	Colocalization with Lamc1 expression in dorsal root ganglion extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: lumbar dorsal root ganglion	Strong	Regionally restricted	2A	Strong expression in dorsal root ganglion. Heterogeneous expression within lumbar DRG.
TS28: segmental spinal nerve	Strong	Regionally restricted	2A	Heterogeneous expression associated nerve. Strong expression in in sensory afferent fibers. Weaker expression in motor part of nerves emerging from dorsal root. Mixed expression in distal part of nerve where motor and sensory fibers are not segregated.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve dorsal root	Strong	Punctate	2B	Strong expression in sensory afferent fibers. Punctate pattern of expression in purely sensory dorsal roots.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve ventral root	Weak	Punctate	2C	Weaker expression in motor part of nerves. Punctate pattern of expression in motor fibers exiting the spinal cord.

Structure	Level	Pattern	Image	Note
TS28: spinal cord mixed spinal nerve	Strong	Regionally restricted	2D	Strong expression in sensory fibers in part of nerve close to dorsal root ganglion, where sensory and motor fibers are segregated.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve dorsal root	Very strong	Regionally restricted	2E	Strongest expression along sensory fibers of sensory branch more distally, when sensory and motor branches diverge.

Structure	Level	Pattern	Image	Note
TS28: spinal cord mixed spinal nerve	Present	Regionally restricted	2F	Authors report difference between sensory and motor fibers in motor branches when sensory and motor branches diverge.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Strong	Regionally restricted	3B , 3A	Strong expression around unmyelinated small caliber sensory neurons. grouped into Remak bundles, in a uniform manner.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Strong	Regionally restricted	3D , 3C	Expression dramatically increased in myelinated fibers concentrated at nodes of Ranvier, partially colocalizing with Lamc1 expression. No expression along basal lamina covering myelinated figers.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present	Regionally restricted	3C'	Expression at center of node of Ranvier.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present	Regionally restricted	3E , 3F	Expression localized at nodes of Ranvier.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Strong	Regionally restricted	3H , 3G	Strong expression in basal lamina above paranodal region, extending beyond to tregion of node.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present	Regionally restricted	3G'	Expression at center of node of Ranvier.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present	Regionally restricted	3I , 3J	Expression extends close to microvilli and possibly into extracellular space between microvilli.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present	Regionally restricted	3I'	Expression at center of node of Ranvier, alternating with Rdx expression, suggesting expression infiltrates space between microvilli.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present		4A , 4B

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Present	Regionally restricted	4E , 4G	Expression in zones where myelin is absent.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad	Present	Regionally restricted	5A	In glabrous skin, dense expression in dermis around nerve endings.
TS28: metapodial pad epidermis	Present	Regionally restricted	5A	Fine network of expression near basal layers of epiderms.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad epidermis	Present	Regionally restricted	5B	Expression in Schwann cell processes or basal lamina partially covering unmyelinated free nerve endings entering eppidermis.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad	Present	Regionally restricted	5C	Expression around myelinated fibers, known to lose their myelin sheath in their terminal arborization.

Structure	Level	Pattern	Image	Note
TS28: epidermis basal layer	Present	Regionally restricted	5E	In hairy skin, dense expression in specific regions of basal cell layer of epidermis. Expression around sensory structures representing terminal Schwann cell sheaths surrounding Merkel cells.

Structure	Level	Pattern	Image	Note
TS28: dermis	Present	Regionally restricted	5F	Deeper in the dermis, expression in matrix of Ruffini corpuscle - aggregates of Schwann cells intertwined in extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: skin	Present	Regionally restricted	5G	In pilo-neural complexes, expression concentrate at base of lanceolate sensory endings, covering myelinated sensory fibers encircling hair follicle. No expression around palisade-shaped endings.

Structure	Level	Pattern	Image	Note
TS28: skin	Present	Regionally restricted	5I	Expression only around type I terminal Schwann cell. No expression around type II.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present	Regionally restricted	6A	Expression around sensory myelinated fibers innervating muscle spindles.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present	Regionally restricted	6B	Expression in basal lamina of neuromuscular junction.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present		S3B	Expression mainly in olfactory mucosa and part of olfactory bulb.
TS27: olfactory segment of nasal mucosa	Present		S3B	Expression mainly in olfactory mucosa and part of olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: olfactory segment of nasal mucosa	Present	Regionally restricted	S3C	Expression restricted to neuronal environment of lamina propria.

Structure	Level	Pattern	Image	Note
TS28: olfactory segment of nasal mucosa	Present	Regionally restricted	S3D	Expression restricted to neuronal environment of lamina propria.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present		S3E
TS28: main olfactory bulb olfactory nerve layer	Present		S3E
TS28: main olfactory bulb glomerular layer	Absent		S3E
TS28: main olfactory bulb mitral cell layer	Absent		S3E

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present		S3F
TS28: main olfactory bulb olfactory nerve layer	Present		S3F
TS28: main olfactory bulb glomerular layer	Absent		S3F
TS28: main olfactory bulb mitral cell layer	Absent		S3F