Antibody:	anti-heparan sulphate proteolycan
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present	Scattered	2A
TS15: neural fold	Present		2A	Deposition is seen in basement membranes and lateral, but not medial, regions of the base of the primitive streak.
TS15: blood vessel	Present		2A	Expression is associated with the basement membrane.
TS15: hindgut	Present		2A	Expression is associated with the basement membrane.
TS15: basement membrane	Present		2A

Structure	Level	Pattern	Image	Note
TS15: neural ectoderm	Strong	Regionally restricted	3A	A dense deposition is seen at the caudal extent of the neuroepithelium, the region where newly formed epithelium merges with neuroepithelium of the posterior neuropore.
TS15: neuropore	Strong	Regionally restricted	3A	A dense deposition is seen at the caudal extent of the neuroepithelium, the region where newly formed epithelium merges with neuroepithelium of the posterior neuropore.
TS15: blood vessel	Strong		3A	Staining is seen in the basement membrane.
TS15: hindgut	Strong		3A	Staining is seen in the basement membrane.
TS15: notochord	Strong		3A	Staining is seen in the basement membrane.
TS15: basement membrane	Strong		3A
TS15: embryo mesenchyme	Strong		3A

Structure	Level	Pattern	Image	Note
TS16: tail mesenchyme	Present		4C
TS16: neural fold	Present	Non-Uniform	4C	The protein is present at the basal region of the radially organized cells; it is excluded from between the rosette of cells.

Structure	Level	Pattern	Image	Note
TS16: embryo mesenchyme	Present		4D
TS16: basement membrane	Present		4D	Expression is seen in the basement membrane of the hindgut.
TS16: hindgut	Present		4D	Expression is seen in the basement membrane of the hindgut.