About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467789
Gene symbol: Pard6b
Gene name: par-6 family cell polarity regulator beta
Antibody: anti-PARD6B (H-64; sc-67392)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A f/f 4um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-1 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A f/f 11um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-2 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A f/f 20um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-3 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A f/f 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-4 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A cKO 4um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-1 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1A cKO 11um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-2 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1A cKO 20um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-3 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1A cKO 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-4 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1C: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1C Expressed at the apical surface in a slightly later, more elongated tubule, which showed a partially absent and numerous discontinuous lumen. And more than one lumen transversely, appearing multi-layered.
Specimen 5A/A': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: blue - Pard6b; red - alpha-tubulin; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS25: renal vesicle Present 5A' , 5A Expressed at the apical surface, which showed a normal continuous lumen. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5B/B': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: blue - Pard6b; red - alpha-tubulin; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS25: renal vesicle Present 5B' , 5B Expressed at the apical surface, which showed a normal continuous lumen. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5C/C': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: blue - Pard6b; red - alpha-tubulin; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS25: renal vesicle Present 5C , 5C' Expressed at the apical surface, which showed a normal continuous lumen. The midbody was formed apically.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory