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Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467807
Gene symbol: Pard6b
Gene name: par-6 family cell polarity regulator beta
Antibody: anti-PARD6B (H-64; sc-67392)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen S1: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Pard6b; blue - Ncam1 (assay MGI:6467819); red - Cre reporter.
Structure Level Pattern Image Note
TS25: s-shaped body Present S1_left , S1_middle Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen S1 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: s-shaped body Present S1_right Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen S2B f/f 3D: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B upper right Expressed at the apical surface, which showed a normal continuous lumen.
Specimen S2B cKO 3D left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B lower middle Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen S2B cKO 3D right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B lower right Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
MGI 6.24 		The Jackson Laboratory