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Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467808
Gene symbol: Pard6b
Gene name: par-6 family cell polarity regulator beta
Antibody: anti-PARD6B (H-64; sc-67392)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1B: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Pard6b; green - Tjp1 (assay MGI:6467822); blue Ncam1 (assay MGI:6467819).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1B Expressed at the apical surface, which showed a small lumen.
Specimen S2B f/f left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Pard6b; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: renal vesicle Present S2B upper left Expressed at the apical surface.
Specimen S2B cKO left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: red - Pard6b; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: renal vesicle Present S2B lower left Similar expression at the apical surface.
Specimen S3A f/f: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Structure Level Pattern Image Note
TS27: urothelium of ureter Present S3A left Expressed at the apical surface, which showed a normal continuous lumen.
Specimen S3A cKO: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: urothelium of ureter Present S3A right Expressed at the apical surface, which showed a normal continuous lumen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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