Probe:	CVprobeSox10(0256)
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS25: presumptive neural crest derivative of pelvic urethra of male	Present	Widespread	8C	The number of stained cells is not abundant, but they are observed throughout the urethral stroma. They are clustered cranial to the anterior prostatic budding zone, between the anterior mesenchymal pad and the bladder, near the caudal boundary of the ventral mesenchymal pad, and in the muscularis propria of the urethra.

Structure	Level	Pattern	Image	Note
TS25: presumptive neural crest derivative of pelvic urethra of female	Present	Widespread	8D	The number of stained cells is not abundant, but they are observed throughout the urethral stroma. They are clustered near the caudal boundary of the ventral mesenchymal pad and in the muscularis propria of the urethra.

Structure	Level	Pattern	Image	Note
TS25: pelvic ganglion	Strong

Structure	Level	Pattern	Image	Note
TS25: superficial cell layer of dorsal pelvic urethra of male	Absent			No expression in the superficial urothelium.
TS25: intermediate cell layer of dorsal pelvic urethra of male	Absent			No expression in the intermediate urothelium.
TS25: basal cell layer of dorsal pelvic urethra of male	Absent			No expression in the basal urothelium.
TS25: lamina propria of dorsal pelvic urethra of male	Absent			No expression in the lamina propria of mesenchyme nor in the macrophages.
TS25: muscularis mucosa of dorsal pelvic urethra of male	Absent			No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of dorsal pelvic urethra of male	Absent			No expression in the submucosa of mesenchyme.
TS25: muscle layer of dorsal pelvic urethra of male	Present
TS25: superficial cell layer of ventral pelvic urethra of male	Absent			No expression in the superficial urothelium.
TS25: intermediate cell layer of ventral pelvic urethra of male	Absent			No expression in the intermediate urothelium.
TS25: basal cell layer of ventral pelvic urethra of male	Absent			No expression in the basal urothelium.
TS25: lamina propria of ventral pelvic urethra of male	Absent			No expression in the lamina propria of mesenchyme nor in the macrophages.
TS25: muscularis mucosa of ventral pelvic urethra of male	Absent			No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of ventral pelvic urethra of male	Absent			No expression in the submucosa of mesenchyme.
TS25: muscle layer of ventral pelvic urethra of male	Present
TS25: mesenchymal pad of pelvic urethra of male	Absent
TS25: vasculature of pelvic urethra of male	Absent			No expression in the vasculature nor in the pericytes.
TS25: presumptive neural crest derivative of pelvic urethra of male	Present
TS25: epithelium of seminal vesicle	Absent
TS25: lamina propria of seminal vesicle	Absent			No expression in the mesenchymal layer.
TS25: muscle of seminal vesicle	Present
TS25: serosa of seminal vesicle	Absent			No expression in the adventitia.
TS25: vasculature of seminal vesicle	Absent
TS25: epithelium of ejaculatory duct	Absent
TS25: lamina propria of ejaculatory duct	Absent			No expression in the mesenchymal layer.
TS25: muscle of ejaculatory duct	Present
TS25: vasculature of ejaculatory duct	Absent
TS25: prostate epithelium	Absent
TS25: peri-prostatic mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS25: superficial cell layer of dorsal pelvic urethra of female	Absent			No expression in the superficial urothelium.
TS25: intermediate cell layer of dorsal pelvic urethra of female	Absent			No expression in the intermediate urothelium.
TS25: basal cell layer of dorsal pelvic urethra of female	Absent			No expression in the basal urothelium.
TS25: lamina propria of dorsal pelvic urethra of female	Absent			No expression in the lamina propria of mesenchyme nor in the macrophages.
TS25: muscularis mucosa of dorsal pelvic urethra of female	Absent			No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of dorsal pelvic urethra of female	Absent			No expression in the submucosa of mesenchyme.
TS25: muscle layer of dorsal pelvic urethra of female	Present
TS25: superficial cell layer of ventral pelvic urethra of female	Absent			No expression in the superficial urothelium.
TS25: intermediate cell layer of ventral pelvic urethra of female	Absent			No expression in the intermediate urothelium.
TS25: basal cell layer of ventral pelvic urethra of female	Absent			No expression in the basal urothelium.
TS25: lamina propria of ventral pelvic urethra of female	Absent			No expression in the lamina propria of mesenchyme nor in the macrophages.
TS25: muscularis mucosa of ventral pelvic urethra of female	Absent			No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of ventral pelvic urethra of female	Absent			No expression in the submucosa of mesenchyme.
TS25: muscle layer of ventral pelvic urethra of female	Present
TS25: mesenchymal pad of pelvic urethra of female	Absent
TS25: vasculature of pelvic urethra of female	Absent			No expression in the vasculature nor in the pericytes.
TS25: presumptive neural crest derivative of pelvic urethra of female	Present
TS25: epithelium of upper vagina	Absent
TS25: lamina propria of upper vagina	Absent			No expression in the mesenchymal layer.
TS25: muscle of upper vagina	Present
TS25: adventitia of upper vagina	Absent
TS25: vasculature of upper vagina	Absent
TS25: epithelium of sinovaginal bulb	Absent			No expression in the epithelial layer of lower vagina.
TS25: lamina propria of sinovaginal bulb	Absent			No expression in the mesenchymal layer of lower vagina.
TS25: muscle of sinovaginal bulb	Present			Expression in the muscle layer of lower vagina.
TS25: adventitia of sinovaginal bulb	Absent			No expression in the adventitia of lower vagina.
TS25: vasculature of sinovaginal bulb	Absent			No expression in the vasculature of lower vagina.