Gt(ROSA)26Sortm1(CAG-Ncre/vvd*,-vvd*/Ccre,-mKate2)Yzwa
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm1(CAG-Ncre/vvd*,-vvd*/Ccre,-mKate2)Yzwa |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Masayuki Yazawa |
| MGI ID: |
MGI:6470703 |
| Synonyms: |
Gt(ROSA)26Sortm1(CAG-cre*,-mKate2)Yzwa, Rosa26PA-Cre A20 |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm1(CAG-Ncre/vvd*,-vvd*/Ccre,-mKate2)Yzwa page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:292311
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Conditional ready, Inducible, Recombinase, Reporter) |
| Inducer: |
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blue light exposure |
| Mutation: |
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Insertion
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Gt(ROSA)26Sortm1(CAG-Ncre/vvd*,-vvd*/Ccre,-mKate2)Yzwa expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| chicken |
CAG |
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Mutation details: The targeting vector was designed with (from 5' to 3') a ubiquitously expressed CAG (CMV enhancer/chicken beta-actin) promoter, a frt-flanked STOP cassette (containing a neomycin resistance [neo] cassette and a polyadenylation [pA] signal). A loxP-flanked cassette including PA-Cre 3.0 followed by a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and a pA site were placed downstream of the frt-flanked STOP cassette. PA-Cre 3.0 consists of a split Cre (59/60) with each fragment complemented with nMag or pMag (respectively) and a nuclear localization sequence (NLS) (CreN59-nMag-NLS and NLS-pMag-CreC60), separated by P2A self-cleaving peptide sequence (2A). The nMag and pMag are part of Magnets protein derived from the fragments of photoreceptor gene product Vivid (VVD) from a filamentous fungi Neurospora crassa. This floxed cassette is followed by a nuclear-localized red fluorescent protein (mKate2) followed by another pA signal. This targeting vector was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into 129Sv/Ev x C57BL/6 derived embryonic stem (ES) cells (clone A20) and correctly targeted ES cells were injected into blastocysts.
(J:292311)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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CAG
(chicken)
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| Original: |
J:292311 Morikawa K, et al., Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun. 2020 May 1;11(1):2141 |
| All: |
1 reference(s) |
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Analysis Tools
