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Tem1(dre)Okn
Endonuclease-mediated Allele Detail
Summary
Symbol: Tem1(dre)Okn
Name: brachyury, T-box transcription factor T; endonuclease-mediated mutation 1, Hideyuki Okano
MGI ID: MGI:6471913
Synonyms: Brachyury2A-Dre
Gene: T  Location: Chr17:8653255-8661328 bp, + strand  Genetic Position: Chr17, 4.92 cM
Alliance: Tem1(dre)Okn page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:284134
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6NCrlCrlj
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Tem1(dre)Okn expression driven by 1 gene
 
Mutation details: CRISPR-targeting introduced at the end of the coding sequence in exon 8 a GSG (Gly-Ser-Gly)-linker, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, P1-like transducing phage D6 dre recombinase gene, and puromycin resistance cassette flanked by FRT sites (FRT/F3/F5). (J:284134)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: T (mouse)
Summary of all recombinase alleles driven by T.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any T Mutation:  59 strains or lines available
Notes
Lentiviral gene trap injected into zygotes.
ES cells = B6NJ-22(UTR) (C57BL/6NCrlCrlj)
References
Original:  J:284134 Serizawa T, et al., Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Development. 2019 Nov 4;146(21):dev174938
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory