Igfbp2em1(cre/ERT2)Ntang
Endonuclease-mediated Allele Detail
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| Symbol: |
Igfbp2em1(cre/ERT2)Ntang |
| Name: |
insulin-like growth factor binding protein 2; endonuclease-mediated mutation 1, Nan Tang |
| MGI ID: |
MGI:6472828 |
| Synonyms: |
Igfbp2-CreER |
| Gene: |
Igfbp2 Location: Chr1:72863662-72891633 bp, + strand Genetic Position: Chr1, 36.94 cM
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| Alliance: |
Igfbp2em1(cre/ERT2)Ntang page
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| Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Igfbp2em1(cre/ERT2)Ntang expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Igfbp2 (MGI:96437) |
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Mutation details: The allele have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the insulin-like growth factor binding protein 2 locus (Igfbp2) on chromosome 1. The targeting vector contained, from 5' to 3', an IRES enhancer region, a CreER(T2) fusion gene (Cre-ER(T2); Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), and an SV40 polyadenylation sequence. The plasmid was targeted to the 3' UTR of the endogenous mouse Igfbp2 gene via CRISPR/Cas9 mediated gene targeting in C57BL/6N-derived zygotes. Offspring were bred with FVB mice for germline transmission of the allele. The resulting Igfbp2-CreER colony was then backcrossed to FVB mice for at least 6 generations prior to sending males.
(J:260595)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Igfbp2
(mouse)
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| Original: |
J:260595 Wang Y, et al., Pulmonary alveolar type I cell population consists of two distinct subtypes that differ in cell fate. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2407-2412 |
| All: |
1 reference(s) |
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Analysis Tools
