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Gfi1tm2.1(GFP/cre/ERT2)Gan
Targeted Allele Detail
Summary
Symbol: Gfi1tm2.1(GFP/cre/ERT2)Gan
Name: growth factor independent 1 transcription repressor; targeted mutation 2.1, Lin Gan
MGI ID: MGI:6477635
Synonyms: Gfi1-GCE
Gene: Gfi1  Location: Chr5:107864521-107873671 bp, - strand  Genetic Position: Chr5, 52.23 cM
Alliance: Gfi1tm2.1(GFP/cre/ERT2)Gan page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:297267
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEv or C57BL/6J
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase, Reporter)
Inducer:    tamoxifen
Mutation:    Insertion
 
Gfi1tm2.1(GFP/cre/ERT2)Gan expression driven by 1 gene
 
Mutation details: The coding sequence for P2A-GFP/cre/ERT2 and a downstream FRT-flanked neomycin resistance cassette was inserted in-frame to the C-terminus resulting in bicistronic expression of the endogenous transcript and a tamoxifen-inducible cre recombinase with green fluorescent protein reporter. Breeding to a FLP deleter excised the neo and Immunolabelling of P0 cochlea shows normal nuclear localization of GFI1 and cytoplasmic expression of GFP/cre/ERT2, which migrates to the nucleus after tamoxifen administration. This allele expresses a high level of tamoxifen-inducible cre specific to developing hair cells of the vestibule and cochlea. (J:297267)
Inheritance:    Not Specified
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gfi1 (mouse)
Summary of all recombinase alleles driven by Gfi1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 189 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gfi1 Mutation:  31 strains or lines available
References
Original:  J:297267 Tang Q, et al., Gfi1-GCE inducible Cre line for hair cell-specific gene manipulation in mouse inner ear. Genesis. 2019 Sep;57(9):e23304
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory