Antibody:	anti-collagen VI
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The antibody used in this assay recognizes the heterotrimer.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		3A

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	3A	Expression is seen in the blood vessels.

Structure	Level	Pattern	Image	Note
TS28: myometrium	Present

Structure	Level	Pattern	Image	Note
TS13: neural tube	Absent		3B
TS13: somite	Absent		3B

Structure	Level	Pattern	Image	Note
TS13: future brain	Absent		3C	No expression is seen in anterior neural tissue.
TS13: embryo mesenchyme	Absent		3C

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		3D

Structure	Level	Pattern	Image	Note
TS17: brain	Absent		3E	No expression is seen in anterior neural tissue.
TS17: embryo mesenchyme	Absent		3E

Structure	Level	Pattern	Image	Note
TS13: yolk sac	Absent		3F

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Absent		3G

Structure	Level	Pattern	Image	Note
TS19: yolk sac mesenchyme	Present	Regionally restricted	3H	Expression is seen in the thick layer of extracellular matrix.

Structure	Level	Pattern	Image	Note
TS20: yolk sac mesenchyme	Present	Regionally restricted	3I	Expression is seen in the extracellular matrix.

Structure	Level	Pattern	Image	Note
TS21: yolk sac mesenchyme	Strong	Widespread	3J	Expression is seen in the extracellular matrix throughout the mesoderm layer and around blood vessels.

Structure	Level	Pattern	Image	Note
TS22: yolk sac mesenchyme	Strong	Regionally restricted	3K	Expression is seen in the extracellular matrix throughout the mesoderm layer and around blood vessels.

Structure	Level	Pattern	Image	Note
TS19: head surface ectoderm	Present		4A	Expression is seen in the subectodermal basement membrane region of the branchial arches.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present			Expression is seen in the wall.

Structure	Level	Pattern	Image	Note
TS20: dermis	Present		4B	Expression is seen in the developing dermis (in the developing snout).

Structure	Level	Pattern	Image	Note
TS21: dermis	Present		4C	Expression is seen in the developing dermis (in the developing snout).

Structure	Level	Pattern	Image	Note
TS22: dermis	Present		4D	Expression is seen in the developing dermis (in the developing snout).

Structure	Level	Pattern	Image	Note
TS19: forebrain	Absent		4E
TS19: head mesenchyme	Present		4E	Expression is associated with the subepidermal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Absent		4F
TS20: head mesenchyme	Present		4F	Expression is seen in the mesenchymal layer adjacent to the central nervous system.

Structure	Level	Pattern	Image	Note
TS21: forebrain	Absent		4G
TS21: head mesenchyme	Present		4G	Expression is seen in the mesenchymal layer adjacent to the central nervous system.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Absent		4H
TS22: head mesenchyme	Present		4H	Expression is localized to the mesenchymal layer adjacent to the central nervous system.

Structure	Level	Pattern	Image	Note
TS20: liver	Strong	Non-Uniform	7A	Strong expression is seen in the connective tissue capsule encasing the hepatic lobes; low levels are seen throughout the liver.

Structure	Level	Pattern	Image	Note
TS21: liver	Strong	Non-Uniform	7B	Strong expression is seen in the connective tissue capsule encasing the hepatic lobes; low levels are seen throughout the liver.

Structure	Level	Pattern	Image	Note
TS22: liver	Strong	Non-Uniform	7C	Strong expression is seen in the connective tissue capsule encasing the hepatic lobes; low levels are seen throughout the liver.

Structure	Level	Pattern	Image	Note
TS20: heart	Ambiguous		7D	There is no or low levels of staining in the myocardium and pericardium in the atrium and ventricular regions.

Structure	Level	Pattern	Image	Note
TS21: heart	Ambiguous		7E	There is no or low levels of staining in the myocardium and pericardium in the atrium and ventricular regions.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		7F

Structure	Level	Pattern	Image	Note
TS21: brain blood vessel	Present		7G	Expression is seen around the brain capillaries.

Structure	Level	Pattern	Image	Note
TS22: brain blood vessel	Present		7H	Expression is seen around the brain capillaries.

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Strong	Regionally restricted	7I	Expression is seen in the smooth muscle layer.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Weak			Low levels of expression are associated with small blood vessels in embryonic tissues.

Structure	Level	Pattern	Image	Note
TS20: perichondrium	Present		9A	Expression is seen in the matrix surrounding the developing rib cartilage but was not a component of the chondrocyte matrix.
TS20: rib pre-cartilage condensation	Absent		9A	Expression is seen in the matrix surrounding the developing rib cartilage but was not a component of the chondrocyte matrix.

Structure	Level	Pattern	Image	Note
TS21: perichondrium	Present	Regionally restricted	9B	Expression is seen in the matrix surrounding the developing rib cartilage but was not a component of the chondrocyte matrix.
TS21: rib cartilage condensation	Absent		9B	Expression is seen in the matrix surrounding the developing rib cartilage but was not a component of the chondrocyte matrix.

Structure	Level	Pattern	Image	Note
TS22: rib cage	Present	Regionally restricted	9C	Pericellular staining is seen in the chondrocytes.
TS22: perichondrium	Present		9C	Expression is seen in the matrix surrounding the developing rib cartilage.
TS22: rib cartilage condensation	Absent		9C	Expression is seen in the matrix surrounding the developing rib cartilage.

Structure	Level	Pattern	Image	Note
TS21: forelimb cartilage condensation	Present	Regionally restricted	9D	Pericellular and perichondrial staining was seen in cartilage of developing long bones.
TS21: perichondrium	Weak		9D	Pericellular and perichondrial staining was seen in cartilage of developing long bones.

Structure	Level	Pattern	Image	Note
TS21: forelimb muscle	Strong		9E

Structure	Level	Pattern	Image	Note
TS22: forelimb cartilage condensation	Strong	Regionally restricted	9F	Expression is seen in the perichondrial region of developing limbs.
TS22: perichondrium	Strong	Regionally restricted	9F	Expression is seen in the perichondrial region of developing limbs.

Structure	Level	Pattern	Image	Note
TS22: intervertebral disc	Strong		9G	Expression is seen in the intervertebral matrix of the vertebral column

Structure	Level	Pattern	Image	Note
TS20: gut	Absent
TS20: lung	Absent

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		11A	Expression is localized to the basement membrane and the mesenchyme of interbud regions of the lung; it is markedly reduced or absent in the basement membrane and surrounding mesenchymal matrix around distal bud regions.
TS22: trachea	Strong	Regionally restricted	11A	Intense staining is seen in the basement membrane region around the trachea.

Structure	Level	Pattern	Image	Note
TS22: gut mesenchyme	Present	Uniform	11B	No staining is seen in the subepithelial basement membrane.

Structure	Level	Pattern	Image	Note
TS22: lateral ventricle choroid plexus	Ambiguous		11C	Expression is absent or very low in the choroid plexus mesenchyme.
TS22: forebrain meninges	Present		11C	Expression is present in the meningeal extracellular matrix adjacent to the neural tissue.

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle choroid plexus	Ambiguous		11D	Expression is absent or very low in the choroid plexus mesenchyme.
TS22: hindbrain meninges	Strong		11D	Expression is seen in the mesenchyme around the neural tissue adjacent to the choroid plexus, the meningeal extracellular matrix adjacent to the neural tissue.