Probe:	Npn2 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present		1B	Expression only in head mesenchyme.

Structure	Level	Pattern	Image	Note
TS12: future brain neural fold	Strong		1H	Abundant expression in neural folds of both head and trunk.
TS12: future spinal cord neural fold	Strong		1H	Abundant expression in neural folds of both head and trunk.
TS12: somite	Present	Regionally restricted	1H	Expression in mesoderm of somites.

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present		1I	Expression only in head mesenchyme.

Structure	Level	Pattern	Image	Note
TS12: future brain neural fold	Strong		1J	Abundant expression in neural folds of head.
TS12: head mesenchyme	Present		1J	Expression persists in head mesenchyme.

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural fold	Strong		1K	Abundant expression in neural folds of trunk.

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Strong		1P	Prominent stripe of expression at midbrain hindbrain boundary.
TS13: future brain neural crest	Present	Regionally restricted	1P	Expression in migrating cranial neural crest, dorsal neural tube and neural folds before migration
TS13: future spinal cord neural fold	Present	Regionally restricted	1P	Expression in migrating cranial neural crest, dorsal neural tube and neural folds before migration
TS13: migrating neural crest	Present	Regionally restricted	1P	Expression in migrating cranial neural crest, dorsal neural tube and neural folds before migration
TS13: neural tube	Present	Regionally restricted	1P	Expression in migrating cranial neural crest, dorsal neural tube and neural folds before migration

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present	Regionally restricted	1Q	Expression in migrating cranial neural crest.
TS13: migrating neural crest	Present	Regionally restricted	1Q	Expression in migrating cranial neural crest.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	1R	Expression in dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural fold	Present	Regionally restricted	1S	Expression in neural folds before migration.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	1V	Expression in first (rhombomere 2) and second (rhombomere 4) branchial arch of migrating neural crest cells.
TS14: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	1V	Expression in first (rhombomere 2) and second (rhombomere 4) branchial arch of migrating neural crest cells.
TS14: future hindbrain neural crest	Present	Regionally restricted	1V	Expression in first (rhombomere 2) and second (rhombomere 4) branchial arch of migrating neural crest cells.

Structure	Level	Pattern	Image	Note
TS14: midbrain-hindbrain junction	Strong		2A	Prominent stripe of expression at midbrain-hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	2B	Expression in migrating neural crest cells in periocular region.
TS14: neural tube	Present	Regionally restricted	2B	Expression in dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present		2C
TS15: 2nd branchial arch mesenchyme	Present		2C
TS15: trigeminal preganglion	Present		2C

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2G	Expression in rhombomere 2 stream.

Structure	Level	Pattern	Image	Note
TS14: branchial arch mesenchyme	Present	Regionally restricted	2H	No expression adjacent to rhombomere 3.

Structure	Level	Pattern	Image	Note
TS14: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2I	Expression in rhombomere 4 stream.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present	Regionally restricted	2J	Expression in mesodermal core of first and second branchial arches.
TS15: 2nd branchial arch mesenchyme	Present	Regionally restricted	2J	Expression in mesodermal core of first and second branchial arches.