Probe:	Sema3f probe7
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	2D	Stripes of expression at level of caudal midbrain, and rhombomeres 3 and 5.
TS14: rhombomere 3	Present	Regionally restricted	2D	Stripes of expression at level of caudal midbrain, and rhombomeres 3 and 5.
TS14: rhombomere 5	Present	Regionally restricted	2D	Stripes of expression at level of caudal midbrain, and rhombomeres 3 and 5.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	2E	Expression in broad bands in forebrain and caudal midbrain.
TS14: prosencephalon	Present	Regionally restricted	2E	Expression in broad bands in forebrain and caudal midbrain.

Structure	Level	Pattern	Image	Note
TS15: head	Present	Regionally restricted	2F	Expression in frontal portion of face.
TS15: head surface ectoderm	Present		2F
TS15: pharynx epithelium	Present		2F

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Present		2K

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present		2L

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		2M	Expression stripes in neural tube.
TS15: rhombomere 3	Present		2M	Expression stripes in neural tube.
TS15: rhombomere 5	Present		2M	Expression stripes in neural tube.
TS15: head mesenchyme	Present		2M	Expression extends into cranial mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present	Regionally restricted	2N	Expression in core of first and second branchial arches.
TS15: 2nd branchial arch mesenchyme	Present	Regionally restricted	2N	Expression in core of first and second branchial arches.