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Rpe65tm1.1(cre/ERT2)Kser
Targeted Allele Detail
Summary
Symbol: Rpe65tm1.1(cre/ERT2)Kser
Name: retinal pigment epithelium 65; targeted mutation 1.1, Philip Kiser
MGI ID: MGI:6501720
Gene: Rpe65  Location: Chr3:159304812-159330958 bp, + strand  Genetic Position: Chr3, 82.52 cM, cytoband H4
Alliance: Rpe65tm1.1(cre/ERT2)Kser page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:310003
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Rpe65tm1.1(cre/ERT2)Kser expression driven by 1 gene
 
Mutation details: The targeting construct was designed to fuse a P2A self-cleaving peptide sequence and a cre/ERT2 sequence in frame at the 3' of exon 14 of the retinal pigment epithelium 65 (Rpe65) gene. The cre-ERT2 fusion gene contains a Cre recombinase fused to a human estrogen receptor ligand binding domain. A frt-flanked neomycin resistance (neo) cassette was placed immediately after the non-coding exon 15. This construct was electroporated into C57BL/6 FLP-derived iTL BF1 embryonic stem (ES) cells. The Neo cassette in the targeting sequence was removed during ES clone expansion. (J:310003)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Rpe65 (mouse)
Summary of all recombinase alleles driven by Rpe65.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rpe65 Mutation:  52 strains or lines available
References
Original:  J:310003 Choi EH, et al., An inducible Cre mouse for studying roles of the RPE in retinal physiology and disease. JCI Insight. 2021 May 10;6(9)
All:  5 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory