Antibody:	anti-DISC1 (D27)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1a

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		1c

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present	Widespread	2a	Expression in all layers .
TS28: cortical layer II	Strong		2a	Higher intensity of expression in layers II-III and V-VI.
TS28: cortical layer III	Strong		2a	Higher intensity of expression in layers II-III and V-VI.
TS28: cortical layer V	Strong		2a	Higher intensity of expression in layers II-III and V-VI.
TS28: cortical layer VI	Strong		2a	Higher intensity of expression in layers II-III and V-VI.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Scattered	2b	Expression in isolated cell bodies scattered throughout whole cortex.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Widespread	2c	Expression in all regions
TS28: hippocampus CA3	Strong		2c	Higher intensity of expression in CA3 region.

Structure	Level	Pattern	Image	Note
TS28: CA3 pyramidal cell layer	Strong	Clusters	2d	Highest density of expression in pyramidal cells of CA3 region. Uneven clustered pattern of intracelluar distribution.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Widespread

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Strong	Widespread	2e	High expression through whole region.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Strong	Clusters	2f	Expression intense throughout. Uneven clustered pattern of intracelluar distribution.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2g
TS28: cerebellum granule cell layer	Present		2g
TS28: cerebellum molecular layer	Present		2g

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Clusters	2h	Purkinje cells predominantly positive for expression. Uneven clustered pattern of intracelluar distribution.
TS28: cerebellum granule cell layer	Present	Single cells	2h	Some expression in other small cells in molecular layer and granule layer.
TS28: cerebellum molecular layer	Present	Single cells	2h	Some expression in other small cells in molecular layer and granule layer.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Regionally restricted	2i	Strong expression in isolated neurons in dicrete region of brainstem, including spinal triminal nucleus, lateral paragigantocellular nuclue and superior vestibular nucleus.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Clusters	2j	Uneven clustered pattern of intracelluar distribution.

Structure	Level	Pattern	Image	Note
TS17: embryo	Trace		7a	Very low, almost negligible, expression level.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		7b	High expression in very discrete areas of brain including olfactory area, developing cortex, hypothalamus and pons.
TS22: hypothalamus	Present		7b	High expression in very discrete areas of brain including olfactory area, developing cortex, hypothalamus and pons.
TS22: olfactory lobe	Present		7b	High expression in very discrete areas of brain including olfactory area, developing cortex, hypothalamus and pons.
TS22: pons	Present		7b	High expression in very discrete areas of brain including olfactory area, developing cortex, hypothalamus and pons.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present		7b inset upper	Many expressing cells in cortical layer.

Structure	Level	Pattern	Image	Note
TS22: olfactory lobe	Present	Single cells	7b inset lower	Discrete number of isolated expressing cells.

Structure	Level	Pattern	Image	Note
TS22: heart	Present			Expression in peripheral tissue such as heart and liver.
TS22: liver	Present			Expression in peripheral tissue such as heart and liver.

Structure	Level	Pattern	Image	Note
TS25: midbrain	Present	Regionally restricted	7c	Authors report adult-like pattern of expression. Expression in intermediate zone of wall of lateral ventricle and midbrain area.
TS25: telencephalon	Present	Regionally restricted	7c	Authors report adult-like pattern of expression. Expression in intermediate zone of wall of lateral ventricle and midbrain area.

Structure	Level	Pattern	Image	Note
TS25: neocortex	Strong		7c inset	Strong expression in neopallial cortex.