Probe:	ectodin cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: early bud stage tooth	Present	Regionally restricted	1A	Expressed in the underlying mesenchyme of lower incisor region.

Structure	Level	Pattern	Image	Note
TS21: lower jaw incisor mesenchyme	Present	Regionally restricted	1C	Increased expression in the underlying mesenchyme of lower incisor region.

Structure	Level	Pattern	Image	Note
TS22: incisor epithelium	Present	Regionally restricted	1E	Expression was absent from the enamel knot.
TS22: incisor dental papilla	Strong		1E
TS22: incisor mesenchyme	Strong	Regionally restricted	1E	Expressed in the mesenchyme at the lingual side of the incisor. No expression in the mesenchyme under the enamel knot.
TS22: enamel knot	Absent		1E

Structure	Level	Pattern	Image	Note
TS22: incisor epithelium	Present	Regionally restricted	S5E	Expression was absent from the enamel knot.
TS22: incisor dental papilla	Strong		S5E
TS22: incisor mesenchyme	Strong	Regionally restricted	S5E	Expressed in the mesenchyme at the lingual side of the incisor. No expression in the mesenchyme under the enamel knot.
TS22: enamel knot	Absent		S5E

Structure	Level	Pattern	Image	Note
TS22: incisor epithelium	Present		S5F	Expression was wider compared with the wild-type.
TS22: incisor dental papilla	Present		S5F	Expression was weaker compared with the wild-type.
TS22: incisor mesenchyme	Present		S5F	Broadly similar to that in wild-type teeth.
TS22: enamel knot	Absent		S5F