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Chattm1.1(flpo)Rmcld
Targeted Allele Detail
Summary
Symbol: Chattm1.1(flpo)Rmcld
Name: choline O-acetyltransferase; targeted mutation 1.1, Robert Machold
MGI ID: MGI:6512860
Synonyms: ChatFlpO
Gene: Chat  Location: Chr14:32130160-32187866 bp, - strand  Genetic Position: Chr14, 19.4 cM
Alliance: Chattm1.1(flpo)Rmcld page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:303249
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Chattm1.1(flpo)Rmcld expression driven by 1 gene
 
Mutation details: The targeting vector containing an internal ribosome entry site (IRES) fused to a FlpO recombinase sequence and a polyadenylation site was inserted into the 3' UTR of the choline acetyltransferase (Chat) gene. The construct also included a loxP-flanked neomycin resistance cassette. Chimeric mice were bred with CMV-cre mice to remove the neo cassette. (J:303249)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Chat (mouse)
Summary of all recombinase alleles driven by Chat.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chat Mutation:  58 strains or lines available
References
Original:  J:303249 Allaway KC, et al., Cellular birthdate predicts laminar and regional cholinergic projection topography in the forebrain. Elife. 2020 Dec 23;9:e63249
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory