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Ctxn3em1(flpo)Tasic
Endonuclease-mediated Allele Detail
Summary
Symbol: Ctxn3em1(flpo)Tasic
Name: cortexin 3; endonuclease-mediated mutation 1, Bosiljka Tasic
MGI ID: MGI:6514362
Synonyms: Ctxn3em1(flpo)Hze
Gene: Ctxn3  Location: Chr18:57601588-57611205 bp, + strand  Genetic Position: Chr18, 32.15 cM
Alliance: Ctxn3em1(flpo)Tasic page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Ctxn3em1(flpo)Tasic expression driven by 1 gene
 
Mutation details: CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence, a FlpO recombinase gene, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site immediately following the STOP codon. The guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome. (J:101977)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Ctxn3 (mouse)
Summary of all recombinase alleles driven by Ctxn3.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ctxn3 Mutation:  6 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
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