Gpr139tm1.1(flpo)Tasic
Targeted Allele Detail
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| Symbol: |
Gpr139tm1.1(flpo)Tasic |
| Name: |
G protein-coupled receptor 139; targeted mutation 1.1, Bosiljka Tasic |
| MGI ID: |
MGI:6514363 |
| Synonyms: |
Gpr139tm1.1(flpo)Hze |
| Gene: |
Gpr139 Location: Chr7:118739970-118783761 bp, - strand Genetic Position: Chr7, 63.79 cM
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| Alliance: |
Gpr139tm1.1(flpo)Tasic page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Gpr139tm1.1(flpo)Tasic expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a partial Gpr139 sequence within exon 2 up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a FlpO recombinase gene (a codon-optimized FLPe gene-modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an AttP site. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA) and replace it with the recombined AttB/AttP site (AttL).
(J:101977)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Gpr139
(mouse)
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| Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
| All: |
3 reference(s) |
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Analysis Tools
