Slc17a7em1(flpo)Tasic
Endonuclease-mediated Allele Detail
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| Symbol: |
Slc17a7em1(flpo)Tasic |
| Name: |
solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; endonuclease-mediated mutation 1, Bosiljka Tasic |
| MGI ID: |
MGI:6514364 |
| Synonyms: |
Slc17a7em1(flpo)Hze |
| Gene: |
Slc17a7 Location: Chr7:44813373-44825566 bp, + strand Genetic Position: Chr7, 29.16 cM
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| Alliance: |
Slc17a7em1(flpo)Tasic page
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| Allele Type: |
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Endonuclease-mediated (Recombinase) |
| Mutation: |
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Insertion
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Slc17a7em1(flpo)Tasic expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence), a FlpO recombinase gene, a woodchuck hepatitis virus post-transcriptional regulatory element, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site following the STOP codon. The guide vector contained the Slc17a7 guide RNA sequences designed for the endogenous stop codon and also a DNA sequence encoding a human codon-optimized Streptococcus pyogenes CRISPR associated protein 9 (SpCas9). Of note, the guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome.
(J:101977)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Slc17a7
(mouse)
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| Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
| All: |
2 reference(s) |
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Analysis Tools
