Probe:	npn2 insitu probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	3A	Expression is ventral.
TS15: neural tube floor plate	Present		3A
TS15: somite	Present		3A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	3B	Expression is ventral and dorsal.
TS17: dorsal root ganglion	Present	Regionally restricted	3B
TS17: neural tube floor plate	Present	Regionally restricted	3B

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Strong	Regionally restricted	3C/D	Expression is in the region of motoneurons.
TS19: future spinal cord	Strong	Regionally restricted	3C/D	Expression is ventral and dorsal.
TS19: dorsal root ganglion	Strong	Regionally restricted	3C/D
TS19: neural tube floor plate	Strong	Regionally restricted	3C/D
TS19: neural tube roof plate	Present		3C/D

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	3E	Expression is in the entire ventral spinal cord, with stronger expression in patches (likely motoneurons).
TS21: ventral grey horn	Strong	Patchy	3E
TS21: dorsal root ganglion	Trace		3E
TS21: dorsal grey horn	Absent		3E

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	3F	Expression is in the entire ventral spinal cord, with stronger expression in patches (likely motoneurons).
TS23: ventral grey horn	Present	Patchy	3F
TS23: dorsal grey horn	Absent		3F
TS23: dorsal root ganglion	Absent		3F

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Strong		4A/B
TS21: dorsal aorta	Present		4A/B
TS21: spinal cord	Present		4A/B

Structure	Level	Pattern	Image	Note
TS23: sympathetic ganglion	Strong		4C

Structure	Level	Pattern	Image	Note
TS23: inner ear	Strong		4D
TS23: superior cervical ganglion	Strong		4D

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Strong		5A
TS21: vomeronasal organ	Strong		5A

Structure	Level	Pattern	Image	Note
TS23: vomeronasal organ	Strong		5B

Structure	Level	Pattern	Image	Note
TS27: accessory olfactory bulb	Strong		5C
TS27: anterior olfactory nucleus	Strong		5C
TS27: amygdala	Present		5C
TS27: frontal cortex	Present		5C
TS27: hippocampus	Present		5C

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		5D	Expression is stronger in the most rostral regions.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		5E	Expression is stronger in the most rostral regions.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present	Regionally restricted	6A	Expression is in migrating cells.
TS21: head mesenchyme	Present	Regionally restricted	6A	Expression is in mesenchymal cells covering the cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		6B	Expression is most highly expressed in its lower region.
TS23: head mesenchyme	Present	Regionally restricted	6B	Expression is in mesenchymal cells covering the cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		6C	Expression is most highly expressed in its lower region.
TS23: head mesenchyme	Present	Regionally restricted	6C	Expression is in mesenchymal cells covering the cortex.
TS23: hippocampus	Present		6C

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present	Regionally restricted	6E	Expression is ventral.
TS27: hippocampus	Present	Regionally restricted	6E	Expression is medial.
TS27: entorhinal cortex	Present	Regionally restricted	6E	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS17: limb	Strong	Regionally restricted	7A	Expression is in the dorsal and to a lesser extent in the ventral aspects.
TS17: future spinal cord	Present		7A

Structure	Level	Pattern	Image	Note
TS23: gut muscularis	Very strong	Regionally restricted	7B	Expression is in the external smooth muscle layer.
TS23: gut epithelium	Present		7B

Structure	Level	Pattern	Image	Note
TS23: detrusor muscle of bladder	Very strong		7C
TS23: gut muscularis	Very strong		7C
TS23: hindlimb bone	Strong		7C
TS23: hindlimb cartilage condensation	Strong		7C
TS23: vertebra	Strong		7C
TS23: main olfactory bulb	Present		7C
TS23: olfactory epithelium	Present		7C
TS23: tongue muscle	Present		7C
TS23: tooth	Present		7C
TS23: spinal cord	Present	Regionally restricted	7C	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS23: lung	Present		7D
TS23: metanephros	Present		7D
TS23: rib	Present		7D
TS23: submandibular gland	Present		7D
TS23: vibrissa	Present		7D
TS23: organ system	Present	Regionally restricted	7D	Expression is in internal organs.
TS23: heart	Absent		7D