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Gls2em1(cre/ERT2)Hzhu
Endonuclease-mediated Allele Detail
Summary
Symbol: Gls2em1(cre/ERT2)Hzhu
Name: glutaminase 2 (liver, mitochondrial); endonuclease-mediated mutation 1, Hao Zhu
MGI ID: MGI:6704902
Synonyms: Gls2-CreER
Gene: Gls2  Location: Chr10:128030504-128045873 bp, + strand  Genetic Position: Chr10, 76.44 cM
Alliance: Gls2em1(cre/ERT2)Hzhu page
Mutation
origin
Strain of Origin:  C57BL/6J
Mutation
description
Allele Type:    Endonuclease-mediated (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Gls2em1(cre/ERT2)Hzhu expression driven by 1 gene
 
Mutation details: The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the glutaminase 2 (liver, mitochondrial) locus (Gls2) on chromosome 10. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission. (J:304477)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gls2 (mouse)
Summary of all recombinase alleles driven by Gls2.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gls2 Mutation:  33 strains or lines available
References
Original:  J:304477 Wei Y, et al., Liver homeostasis is maintained by midlobular zone 2 hepatocytes. Science. 2021 Feb 26;371(6532)
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory