Gene Expression Data
Assay Details
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Assay
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Reference:
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J:300181
Gomez HF, et al., Morphological study of embryonic Chd8(+/-) mouse brains using light-sheet microscopy. BMC Res Notes. 2021 Jan 12;14(1):23
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6715657
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Gene symbol:
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Cdh2
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Gene name:
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cadherin 2
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Antibody:
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anti-N-cadherin (clone 32; 610920/610921)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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Optical clearing was performed using the Clear Unobstructed Brain/Body Imaging Cocktails and Computational Analysis (CUBIC) protocol.
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Results
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Specimen
1a raw:
embryonic day 12.5
(more  )
Specimen
1a raw:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1a raw
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Expression was detected in neuronal epithelial tissue.
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Specimen
1a horizontal slice:
embryonic day 12.5
(more )
Specimen
1a horizontal slice:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1b WT1 raw:
embryonic day 12.5
(more )
Specimen
1b WT1 raw:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw WT1
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Expression was detected in neuronal epithelial tissue.
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Specimen
1b WT2 raw:
embryonic day 12.5
(more )
Specimen
1b WT2 raw:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw WT2
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Expression was detected in neuronal epithelial tissue.
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Specimen
1b HET1 raw:
embryonic day 12.5; Chd8em1Fezh/Chd8 +
(more )
Specimen
1b HET1 raw:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Chd8em1Fezh/Chd8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw HET1
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Expression was detected in neuronal epithelial tissue.
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Specimen
1b HET2 raw:
embryonic day 12.5; Chd8em1Fezh/Chd8 +
(more )
Specimen
1b HET2 raw:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Chd8em1Fezh/Chd8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw HET2
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Expression was detected in neuronal epithelial tissue.
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Specimen
1b HET3 raw:
embryonic day 12.5; Chd8em1Fezh/Chd8 +
(more )
Specimen
1b HET3 raw:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Chd8em1Fezh/Chd8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw HET3
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Expression was detected in neuronal epithelial tissue.
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Specimen
1b HET4 raw:
embryonic day 12.5; Chd8em1Fezh/Chd8 +
(more )
Specimen
1b HET4 raw:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Chd8em1Fezh/Chd8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw HET4
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Expression was detected in neuronal epithelial tissue.
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Specimen
1b HET5 raw:
embryonic day 12.5; Chd8em1Fezh/Chd8 +
(more )
Specimen
1b HET5 raw:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Chd8em1Fezh/Chd8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
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1b raw HET5
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Expression was detected in neuronal epithelial tissue.
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Analysis Tools