Cxcr4tm1.1(cre/ERT2)Stum
Targeted Allele Detail
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| Symbol: |
Cxcr4tm1.1(cre/ERT2)Stum |
| Name: |
C-X-C motif chemokine receptor 4; targeted mutation 1.1, Ralf Stumm |
| MGI ID: |
MGI:6719054 |
| Synonyms: |
Cxcr4CreER, Cxcr4CreER(T2)-IRES-eGFP |
| Gene: |
Cxcr4 Location: Chr1:128515936-128520030 bp, - strand Genetic Position: Chr1, 56.43 cM
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| Alliance: |
Cxcr4tm1.1(cre/ERT2)Stum page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:288822
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
C57BL/6J
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| Allele Type: |
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Targeted (Inducible, Null/knockout, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Cxcr4tm1.1(cre/ERT2)Stum expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Cxcr4 (MGI:109563) |
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Mutation details: The targeting vector consisting of fragments of 2.9 kb, 3.8 kb and 3.3 kb in size containing the distal promoter sequence, the sequence upstream of exon 1, and the sequence downstream of exon 1, respectively, were used to insert a CreER(T2)-IRES-EGFP cassette and an FRT-flanked neomycin cassette in the 5 end of the 3.3-kb fragment. The linearized construct was electroporated into C57BL/6J mouse ES cells. The neomycin cassette was removed by breeding with C57BL/6J mice expressing Flp-recombinase. With CreER inserted into exon 1, Cxcr4CreER does not produce functional Cxcr4 and homozygous mice bearing this allele are fully deficient in Cxcr4, as verified by immunohistochemistry.
(J:288822)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Cxcr4
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Cxcr4 Mutation: |
46 strains or lines available
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| Original: |
J:288822 Werner Y, et al., Cxcr4 distinguishes HSC-derived monocytes from microglia and reveals monocyte immune responses to experimental stroke. Nat Neurosci. 2020 Mar;23(3):351-362 |
| All: |
8 reference(s) |
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Analysis Tools
