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Irs4tm1.1(cre)Dolsn
Targeted Allele Detail
Summary
Symbol: Irs4tm1.1(cre)Dolsn
Name: insulin receptor substrate 4; targeted mutation 1.1, David P Olson
MGI ID: MGI:6720261
Synonyms: IRS4-iCre
Gene: Irs4  Location: ChrX:140493994-140508213 bp, - strand  Genetic Position: ChrX, 62.43 cM
Alliance: Irs4tm1.1(cre)Dolsn page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:299851
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Irs4tm1.1(cre)Dolsn expression driven by 1 gene
 
Mutation details: Using a BAC recombineering method, a targeting construct containing an insertion of an Frt-flanked neomycin selection cassette followed by an internal ribosomal entry (tires) sequence fused to a Cre recombinase transgene (iCre) between the STOP codon and the polyadenylation of the Irs4 gene. This construct was used to isolate a targeting plasmid with the neo-iCre cassette with upstream and downstream Crh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. (J:299851)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Irs4 (mouse)
Summary of all recombinase alleles driven by Irs4.
 

Expression
In Mice Carrying this Mutation: 2 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Irs4 Mutation:  5 strains or lines available
References
Original:  J:299851 Sutton AK, et al., Paraventricular, subparaventricular and periventricular hypothalamic IRS4-expressing neurons are required for normal energy balance. Sci Rep. 2020 Mar 26;10(1):5546
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory