Cd209btm1.1(cre,HBEGF)Bcgen
Targeted Allele Detail
|
|
| Symbol: |
Cd209btm1.1(cre,HBEGF)Bcgen |
| Name: |
CD209b antigen; targeted mutation 1.1, Biocytogen LLC |
| MGI ID: |
MGI:6755214 |
| Synonyms: |
Cd209btm1.1(cre,DTR)Bcgen, SIGN-R1-Cre/DTR |
| Gene: |
Cd209b Location: Chr8:3967655-3976841 bp, - strand Genetic Position: Chr8, 1.92 cM, cytoband A1.2-A1.3
|
| Alliance: |
Cd209btm1.1(cre,HBEGF)Bcgen page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:288614
|
| Parent Cell Line: |
Not Specified (ES Cell)
|
| Strain of Origin: |
C57BL/6
|
|
| Allele Type: |
|
Targeted (Inserted expressed sequence, Recombinase) |
| Mutation: |
|
Insertion
|
| |
|
Cd209btm1.1(cre,HBEGF)Bcgen expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
HBEGF (1839) |
|
|
|
| |
|
Cd209btm1.1(cre,HBEGF)Bcgen expression driven by
1 gene
Knock-in expression driven by:
|
| |
|
Mutation details: A cDNA construct encoding an Frt-flanked neomycin resistance cassette followed by a stop cassette was introduced before a start codon, followed by cDNA encoding Cre recombinase, and the human DTR (HBEGF) was generated. The Cre recombinase and DTR coding sequences were separated by self-cleaving 2A viral sequences to allow stoichiometric translation of both individual proteins. This construct was inserted at the ATG start codon of cd209b, effectively replacing the expression of SIGN-R1. The neomycin resistance gene and stop cassette was removed by crossing to FLPO mice.
(J:288614)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Cd209b
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Cd209b Mutation: |
30 strains or lines available
|
|
| Original: |
J:288614 Pirgova G, et al., Marginal zone SIGN-R1(+) macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):12295-12305 |
| All: |
1 reference(s) |
|
Analysis Tools
