Adgra3tm1.1(HBEGF,-cre/ERT2)Pac
Targeted Allele Detail
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| Symbol: |
Adgra3tm1.1(HBEGF,-cre/ERT2)Pac |
| Name: |
adhesion G protein-coupled receptor A3; targeted mutation 1.1, Pamela Cowin |
| MGI ID: |
MGI:6759474 |
| Synonyms: |
Adgra3cre |
| Gene: |
Adgra3 Location: Chr5:50117293-50216338 bp, - strand Genetic Position: Chr5, 26.85 cM
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| Alliance: |
Adgra3tm1.1(HBEGF,-cre/ERT2)Pac page
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| Allele Type: |
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Targeted (Inducible, Inserted expressed sequence, Null/knockout, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutations: |
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Insertion, Intragenic deletion
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Adgra3tm1.1(HBEGF,-cre/ERT2)Pac expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
HBEGF (1839) |
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Adgra3tm1.1(HBEGF,-cre/ERT2)Pac expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contains a human diphtheria toxin receptor gene (HBEGF) fused to an enhanced green fluorescent protein (EGFP), a self-cleaving peptide T2A, an FRT-flanked neomycin cassette and cre/ERT2 inserted immediately after the first methionine codon. The construct places the diphtheria toxin receptor, EGFP, and cre/ERT2 under the control of the Adgra3 promoter, and excises 502 bp encompassing 221 bp of exon 1 and part of the following intron to generate a knockout. Mice treated with diptheria toxin die due to expression of HBEGF in vital organs. EGFP is not detected in living cells by immunofluorescence or FACS. Flp-mediated recombination removed the FRT-flanked neo cassette.
(J:309699)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Adgra3
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Adgra3 Mutation: |
71 strains or lines available
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| Original: |
J:309699 Spina E, et al., Gpr125 identifies myoepithelial progenitors at tips of lacrimal ducts and is essential for tear film. bioRxiv. 2020; |
| All: |
2 reference(s) |
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Analysis Tools
