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Gene Expression Data
Assay Details
Assay
Reference: J:265712 Dziedzic SA, et al., ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol. 2018 Jan;20(1):58-68
Assay type: Western blot
MGI Accession ID: MGI:6761337
Gene symbol: Ripk3
Gene name: receptor-interacting serine-threonine kinase 3
Antibody: RIP3 antibody (AHP1797)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Tissues were harvested in NP-40 lysis buffer, then treated with 6M urea to show the insoluble fraction, which is known to occur when cells undergo necroptosis.
Results Image: 7a lower RIPK3
 Sample Information Bands Other Sample Information
Lane AgeStructure 50 kDa Genetic BackgroundMutant Allele(s)Sex
Head +/- 1 E16.5 TS24: head Ambiguous involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Head +/- 2 E16.5 TS24: head Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Head +/+ 1 E16.5 TS24: head Weak involves: C57BL/6J Not Specified
Head -/- 1 E16.5 TS24: head Strong involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Head +/+ 2 E16.5 TS24: head Present involves: C57BL/6J Not Specified
Head -/- 2 E16.5 TS24: head Strong involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Head +/- 3 E16.5 TS24: head Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Head +/- 4 E16.5 TS24: head Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/- 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/- 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/+ 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Not Specified
Body -/- 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Strong involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Body +/+ 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Not Specified
Body -/- 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Strong involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Body +/- 3 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/- 4 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory