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Tg(Sprr2f-cre)2Dcas
Transgene Detail
Summary
Symbol: Tg(Sprr2f-cre)2Dcas
Name: transgene insertion 2, Diego H Castrillon
MGI ID: MGI:6764667
Synonyms: BAC-Sprr2f-Cre
Transgene: Tg(Sprr2f-cre)2Dcas  Location: unknown  
Alliance: Tg(Sprr2f-cre)2Dcas page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Tg(Sprr2f-cre)2Dcas expression driven by 1 gene
 
Mutation details: Using a 189 kb BAC clone (RP23-39I4) containing the entire mouse Sprr2f gene, the cre and neo cassette was inserted into the ATG start codon in exon 2 of the mouse Sprr2f gene via bacterial recombineering method. The neo selection cassette was then excised by transient flippase expression, and the resulting engineered BAC was injected into mouse oocytes for the generation of transgenic founder lines. Five founders had cre insertion, the line 2 was assigned to this transgene in absence of specific line information to distinguish it from Tg(Sprr2f-cre)1Dcas generated by the same creator which showed ectopic cre activity in brain and kidney. This transgene shows specific cre activity only in the uterus and no ectopic expression was observed. (J:282812)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Sprr2f (mouse)
Summary of all recombinase alleles driven by Sprr2f.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:282812 Cuevas IC, et al., Fbxw7 is a driver of uterine carcinosarcoma by promoting epithelial-mesenchymal transition. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25880-25890
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory