Nkx2-2tm6.1(Ccre,Nkk2-2)Suss
Targeted Allele Detail
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| Symbol: |
Nkx2-2tm6.1(Ccre,Nkk2-2)Suss |
| Name: |
NK2 homeobox 2; targeted mutation 6.1, Lori Sussel |
| MGI ID: |
MGI:6780118 |
| Synonyms: |
Nkx2.2cCre |
| Gene: |
Nkx2-2 Location: Chr2:147019466-147036163 bp, - strand Genetic Position: Chr2, 72.63 cM, cytoband H
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| Alliance: |
Nkx2-2tm6.1(Ccre,Nkk2-2)Suss page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:311445
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| Parent Cell Line: |
TL1/TL-1 (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Nkx2-2tm6.1(Ccre,Nkk2-2)Suss expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Nkx2-2 (MGI:97347) |
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Mutation details: A DNA construct with COOH-terminal Cre (cCre)-T2A and Frt-flanked hygro selection cassette were inserted using recombineering into an Nkx2-2 basal exchange vector such that Ccre was fused in-frame with the ATG start codon. The modified Nkx2.2 locus and a hygromycin selection cassette were flanked by Lox66 and Lox2272 elements. This vector was co-injected with a cre-expression vector into ES cells containing an Nkx2-2 (loxed cassette acceptor) allele to generate recombination-mediated cassette exchange targetted allele. Animals were bred to ACTB-Flpe mice to excise the hygromycin marker.
(J:311445)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Nkx2-2
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Nkx2-2 Mutation: |
14 strains or lines available
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| Original: |
J:311445 Gross S, et al., Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential. Am J Physiol Gastrointest Liver Physiol. 2015 Dec 15;309(12):G975-87 |
| All: |
1 reference(s) |
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Analysis Tools
