Idh1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:306194 Ghimire S, et al., PEG3 controls lipogenesis through ACLY. PLoS One. 2021;16(5):e0252354
Assay type:	RT-PCR
MGI Accession ID:	MGI:6780738
Gene symbol:	Idh1
Gene name:	isocitrate dehydrogenase 1 (NADP+), soluble

Probe:	Idh1-pC, Idh1-pD
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR was used to obtain these results; Gapdh was used to normalize expression levels.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
WT-F1	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
CoKO-F1	P adult	TS28: mammary gland	Present (a)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Female	gene dosage of Peg3 is almost zero
WT-F2	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
CoKO-F2	P adult	TS28: mammary gland	Present (b)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Female	gene dosage of Peg3 is almost zero
WT-M1	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Male
CoKO-M1	P adult	TS28: mammary gland	Present (c)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Male	gene dosage of Peg3 is almost zero
WT-M2	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Male
CoKO-M2	P adult	TS28: mammary gland	Present (d)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Male	gene dosage of Peg3 is almost zero

Notes:
(a) Expression levels are increased 2.9 fold in the mutant.
(b) Expression levels are increased 1.5 fold in the mutant.
(c) Expression levels are reduced 70% in the mutant.
(d) Expression levels are reduced 60% in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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