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En1tm1.1(flpo)Jbb
Targeted Allele Detail
Summary
Symbol: En1tm1.1(flpo)Jbb
Name: engrailed 1; targeted mutation 1.1, Jay B Bikoff
MGI ID: MGI:6810135
Synonyms: En1-Flpo, En1::Flpo
Gene: En1  Location: Chr1:120530246-120535719 bp, + strand  Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
Alliance: En1tm1.1(flpo)Jbb page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:313825
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout, Recombinase)
Mutation:    Insertion
 
En1tm1.1(flpo)Jbb expression driven by 1 gene
 
Mutation details: Flpo, a codon-optimized version of Flp recombinase was inserted into the ATG in the 1st exon of the En1 genomic locus. Positive ES cell clones were screened by Southern blot analysis and microinjected into blastocysts, and the resulting chimeric mice were crossed to C57BL/6J females. The neomycin selectable cassette was removed using Protamine::Cre mice. (J:313825)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: En1 (mouse)
Summary of all recombinase alleles driven by En1.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any En1 Mutation:  34 strains or lines available
References
Original:  J:313825 Lane AR, et al., Genetic targeting of adult Renshaw cells using a Calbindin 1 destabilized Cre allele for intersection with Parvalbumin or Engrailed1. Sci Rep. 2021 Oct 6;11(1):19861
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory