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Gene Expression Data
Assay Details
Assay
Reference: J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6863897
Gene symbol: Sfta3
Gene name: surfactant associated 3
Reporter: RFP
Antibody: anti-RFP (Rockland)
Detection system: Secondary antibody
Results
Specimen 1B - E12.5: embryonic day 12.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS20: lung epithelium Present Widespread 1B E12.5
Specimen 1B - E16.5: embryonic day 16.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS24: lung epithelium Present Widespread 1B E16.5
Specimen 1B - E18.5: embryonic day 18.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS26: lung epithelium Present Widespread 1B E18.5 , 1C Merge , 1C NANCI-RFP E18.5 All reporter expressing cells are double labeled.
Specimen 1D - Bronchi: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS28: lobar bronchus epithelium Present Widespread 1D Bronchi Merge , 1D Bronchi NANCI-RFP All reporter expressing cells are double labeled.
Specimen 1D - Small Airways: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS28: bronchiole epithelium Present Widespread 1D Small Airways Merge , 1D Small Airways NANCI-RFP All reporter expressing cells are double labeled.
Specimen 1D - Alveoli: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS28: alveolus epithelium Present Widespread 1D Alveoli NANCI-RFP , 1D Alveoli Merge All reporter expressing cells are double labeled.
Specimen S1B - E12.5: embryonic day 12.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS20: medial ganglionic eminence Present S1B E12.5 Merge , S1B E12.5 NANCI-RFP
TS20: preoptic area Present S1B E12.5 NANCI-RFP , S1B E12.5 Merge Expression is seen in the anterior entopeduncular/preoptic area.
TS20: hypothalamus Present S1B E12.5 Merge , S1B E12.5 NANCI-RFP
TS20: forebrain Present Regionally restricted S1B E12.5 Merge , S1B E12.5 NANCI-RFP Areas of expression include the developing globus pallidus.
Specimen S1B - Hypothalamus: embryonic day 18.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS26: hypothalamus Present S1B E18.5 Hypothalamus Merge , S1B E18.5 Hypothalamus NANCI-RFP All reporter expressing cells are double labeled.
Specimen S1B - Globus Pallidus: embryonic day 18.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS26: globus pallidus Present S1B E18.5 Globus Pallidus Merge , S1B E18.5 Globus Pallidus NANCI-RFP All reporter expressing cells are double labeled.
Specimen S2A: postnatal week 10; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps reporter; green - Nkx2-1 (assay MGI:6863898).
Structure Cell Type Level Pattern Image Note
TS28: thyroid gland epithelial cell of thyroid gland Present Regionally restricted S2A upper NANCIRFP/+ , S2A lower Merge , S2A lower NANCIRFP/+ , S2A upper Merge Expression is seen in the epithelium; all reporter expressing cells are double labeled.
Specimen 1F - Sftpc: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Sftpc (assay MGI:6863924).
Structure Level Pattern Image Note
TS28: lung epithelium Present 1F top merge , 1F top NANCI-RFP Some expressing cells are pulmonary epithelium alveolar type 2 (AT2) cells.
Specimen 1F - Scgb1a1: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 1F center NANCI-RFP , 1F center merge Some expressing cells are secretory cells.
Specimen 1F - Foxj1: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Foxj1 (assay MGI:6863930).
Structure Level Pattern Image Note
TS28: lung epithelium Present 1F bottom merge , 1F bottom NANCI-RFP Some expressing cells are ciliated.
Specimen S3E - Pdpn: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Pdpn (assay MGI:6863931).
Structure Level Pattern Image Note
TS28: lung epithelium Present S3E top NANCI-RFP , S3E top merge Some expressing cells are pulmonary epithelium alveolar type 1 (AT1) cells.
Specimen S3E - Pgp9.5: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Uchl1 (assay MGI:6863932).
Structure Level Pattern Image Note
TS28: lung epithelium Present S3E 2nd down merge , S3E 2nd down NANCI-RFP Some expressing cells are neuroendocrine cells.
Specimen S3E - Muc5ac: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Muc5ac (assay MGI:6863934).
Structure Level Pattern Image Note
TS28: lobar bronchus epithelium Present Single cells S3E 3rd down NANCI-RFP , S3E 3rd down merge Expressing cells in the mainstem bronchi are rare; they are not goblet cells.
Specimen S3E - P63: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Sfta3-ps; green - Trp63 (assay MGI:6863935).
Structure Level Pattern Image Note
TS28: lobar bronchus epithelium Present Single cells S3E bottom NANCI-RFP , S3E bottom merge Expressing cells in the mainstem bronchi are rare; they are basal cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
MGI 6.24 		The Jackson Laboratory